Author(s):
Shweta Vinod Raul, Sunila A Patil
Email(s):
Shwetaraul10@gmail.com
DOI:
10.52711/2231-5675.2026.00047 
Address:
Shweta Vinod Raul1*, Sunila A Patil2
1P.S.G.V.P Mandal’s College of Pharmacy, Shahada, Dist. Nandurbar, Maharashtra, India.
2Assistant Professor, Department of Pharmaceutical Quality Assurance, P.S.G.V.P Mandal’s College of Pharmacy, Shahada, Dist. Nandurbar, Maharashtra, India.
*Corresponding Author
Published In:
Volume - 16,
Issue - 4,
Year - 2026
ABSTRACT:
In recent years, ultra-high-pressure liquid chromatography (UHPLC) has become the preferred modern HPLC platform. UHPLC is the ideal tool for rapidly developing techniques since it takes less time for column equilibration and analysis. This paper provides a comprehensive analysis of the status, benefits, and limitations of UHPLC in technique development. Ultra-high-pressure liquid chromatography (UHPLC) has emerged as the contemporary standard HPLC platform in recent years. UHPLC is perfect for rapid method development because of its shorter analytical time and faster column equilibration. The present state, advantages, and drawbacks of UHPLC in technique development are critically reviewed in this article. We highlight recent developments and recommended practices via case examples A contemporary method that provides liquid chromatography a new direction is UPLC. Ultra-Performance Liquid Chromatography (UPLC) is a technique that improves in three key areas: “speed, resolution, and sensitivity. “For particles smaller than 2 ums in diameter, ultra-performance liquid chromatography (UPLC) can be used to improve resolution. When compared to high-performance liquid chromatography (HPLC), speed, and sensitivity.
Cite this article:
Shweta Vinod Raul, Sunila A Patil. Ultra-High-Performance Liquid Chromatography: A Next-Generation Analytical Tool. Asian Journal of Pharmaceutical Analysis. 2026; 16(4):307-0. doi: 10.52711/2231-5675.2026.00047
Cite(Electronic):
Shweta Vinod Raul, Sunila A Patil. Ultra-High-Performance Liquid Chromatography: A Next-Generation Analytical Tool. Asian Journal of Pharmaceutical Analysis. 2026; 16(4):307-0. doi: 10.52711/2231-5675.2026.00047 Available on: https://www.ajpaonline.com/AbstractView.aspx?PID=2026-16-4-9
REFERENCES:
1. Hodgkin K (1985) Towards Earlier Diagnosis. A Guide to Primary Care. Churchill Livingstone.
2. Chopade SV, Patil VR. Introduction to New chromatography technique – UPLC. Available from: https://www.pharma Tutor.org/articles/new-chromatographictechnique-uplc-ultra-performanceliquid-chromatography [Accessed: December 13, 2021].
3. Gumustas M, Kurbanoglu S, Uslu B, Ozkan SA. UPLC versus HPLC on drug Analysis: Advantageous, applications and Their validation parameters. Chromatographia. 2013; 76: 1365-1427.DOI: 10.1007/s10337-013-2477-8
4. Priya Sadapha, Kavita Dhamak. Review Article on High-Performance Liquid Chromatography (HPLC) Method Development and Validation. Int. J. Pharm. Sci. Rev. Res. 2022; 74(2): 23-29. DOI link: http://dx.doi.org/10.47583/ijpsrr.2022.v74i02.003
5. Thomas H. Walter, Richard W. Andrews, Recent innovations in UHPLC columns and instrumentation, Trends in Analytical Chemistry. 2014. Doi: 10.1016/j.trac.2014.07.016
6. Klimczak I, Gliszczynska wiglo A. Comparison of UPLC and HPLC Methods for determination of vitamin C. Food Chemistry. 2015; 175:100-105. DOI: 10.1016/j.foodchem.2014.11.104
7. Komaroff AL. ‘Minor’ illness symptoms: the magnitude of their burden and of our ignorance. Arch Intern Med. 1990; 150: 1586–1587.
8. Sackett DL, Haynes BR, Tugwell P, Guyatt GH (1991) Clinical Epidemiology: a Basic Science for Clinical Medicine. London: Lippincott, Williams and Wilkins.
9. Mullan F. Community-oriented primary care: epidemiology’s role in the future of primary care. Public Health Rep. 1984; 99: 442–445
10. Kunal Bhattacharya, Nongmaithem Randhoni Chanu, Atanu hattacharjee, Bhargab Jyoti Sahariah, Chanam Melody Devi, Ripunjoy Bordoloi. Ultra-Performance Liquid Chromatography –An Updated Review. Research Journal of Pharmacy and Technology. 2022; 15(12): 5849-3. Doi: 10.52711/0974-360X.2022.00987
11. Singh S. A brief review on ultra-Performance liquid chromatography. World Journal of Pharmacy Science. 2015; 6(15): 407-422. DOI: 10.20959/Wjpr201715-10136.
12. Chawla G, Ranjan C. Principle, Instrumentation, and applications of UPLC: A novel technique of liquid Chromatography. Open Chemistry Journal. 2016; 3(1): 1-16. DOI: 10.2174/1874842201603010001
13. Kumar A, Saini G, Nair A, Sharma R. Review uplc: a preeminent technique in pharmaceutical analysis. Acta Poloniae Pharmaceutica. 2012; 69(3): 371-380.
14. Pooja Popat Nagare, Gauri Sitaram Maske. A Brief Review: UHPLC. International Journal of Creative Research Thoughts (IJCRT). 2022; 10(9).
15. k.G. Sheliya, K. V. Shah. Ultra-performance liquid chromatography (UPLC): A modern chromatography technique. Pharma Science Monitor an International Journal of Pharmaceutical Sciences. 2013; 4(3).
16. M. Eswarudu, M. Chinna Eswaraiah et al. Ultra-Performance Liquid Chromatography (UPLC): A Preeminent Technique in Pharmaceutical Analysis. Research J. Pharm. And Tech. 2012; 5(12): 1484-1489.
17. UHPLC Instruments. Davy Guillanne, Jean-Luc Veuthey Leap. University of Geneva
18. Goswami S. UPLC TM-A review on Its recent advances in instrumental Analysis UPLC TM-A review on its Recent advances in instrumental analysis. Indian Journal of Natural Sciences. 2021;12(68): 1-18
19. Bhanot D. What are the Benefits of UHPLC over normal Analytical HPLC? [Internet]. 2014. Available from: https://Lab-training.com/2014/04/17/what-arethe-benefits-of-uhplc-over-normal-ana Lytical-hplc/ [Accessed: December 2021]
20. Gumustas M, Kurbanoglu S, Uslu B, Ozkan SA. UPLC versus HPLC on drug Analysis: Advantageous, applications and Their validation parameters. Chromatographia. 2013; 76: 1365-1427.DOI: 10.1007/s10337-013-2477-8
21. Patel Niti Bharatbhai, Jasmina Surati et al., A Review on UHPLC Instrumentation with Advancements. Journal of Advances in Pharmaceutical Sciences. 2023; 1(1): 33-44 2023
22. Samatha et al. Ultra-Performance Liquid Chromatography (UPLC). World Journal of Pharmacy and Pharmaceutical Sciences. 2015; 4(8); 356-367.
23. Swetha Sri R, Bhavya Sri. K, Mounika. Ch. A Review on Comparative study of HPLC and UPLC. Research J. Pharm. And Tech. 2020: 13(3): 1570-1574. Dok 10.5958/0974-360X.2020. 00284.Χ
24. Mullan F, Nutting PA. Primary care epidemiology: new uses of old tools. Fam Med. 1986; 18: 221–225.
25. Abramson JH. Application of epidemiology in community oriented primary care. Public Health Rep. 1984; 99: 437–441.